Ouchterlony Double Immunodiffusion Method and Equine Grass Sickness

A number of authors have hypothesised that immunity to the cause of Grass Sickness is developed in some horses. Authors investigating the Clostridium botulinum hypothesis demonstrated an increased level of systemic antibodies against the surface antigen of C. botulinum in surviving chronic cases compared to non surviving chronic cases, suggesting that antibody status may not only influence disease development but also the likelihood of survival.

Oucterlony Double Immunodiffusion Method and Grass Sickness